Job Description

Position Objective: Provide services as a Biologist in support of the overall functions of the Vaccine Research Center (VRC), Vaccine Production Program (VPP) within the National Institute of Allergy and Infectious Diseases (NIAID).
Duties And Responsibilities
Contribute to the generation of stable cell lines for vaccine and therapeutic protein candidates including monoclonal antibodies, recombinant proteins, and virus-like particles.
Prepare plasmid DNA using standard molecular cloning techniques including PCR, restriction digest, and bacterial culture.
Independently execute each step of the cell line development process:
Transfection
Pool generation
Single cell cloning
Clone expansion in static culture plates and shake flasks
Fed-batch analysis
Clone stability studies
Use automated platforms for clone selection and screening.
Participate in cell line development platform design, optimization, and cycle-time reduction studies including methods for clone selection, expansion, ranking, and characterization.
Perform small-scale process development studies using various culture vessels including tissue culture plates, shake flasks, and small-scale bioreactors.
Generate stable pools and perform transient protein production for supply of reagent-grade material
Independently complete experiments and analyze data with minimal supervision.
Provide laboratory support for medium preparation and ordering of supplies.
Write and review technical reports documenting process development studies and stable cell line generation work.
Work with other team members to generate experimental plans for process development studies.
Write and review standard operating procedures for cell line development processes and instrumentation.
Maintain complete documentation records, including a detailed and up-to-date laboratory notebook, to support regulatory filings.
Coordinate cell line development projects in collaboration with the project lead and Federal Task Leader:
Plan experiments to fit within required timelines
Communicate any issues or delays promptly and help to identify solutions
Maintain cell line development master sheet file with all critical information and data
Regularly communicate progress to project lead and present work to cell line development team.
Participate in project meetings.
Contribute results and studies for publication in peer-reviewed journals.
Maintain broad knowledge of state-of-the-art principles.

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